Assessment of Antibacterial Efficacy of Trachyspermum Ammi and Piper Nigrum
Keywords:
Trachyspermum ammi, Piper nigrum, Klebsiella pneumoniae, Ampicillin, Gentamicin, Norfloxacin, Streptomycin, Antibacterial activityAbstract
Spices have a significant role in maintaining the wellbeing of human kind. Trachyspermum ammi and Piper
nigrum has the potency to be used as an alternative source as antibacterials. They stand against various bacterial pathogens Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and has been employed in traditional medicine for centuries. It was found to have competitive effects compared to antibiotics Ampicillin, Gentamicin, Norfloxacin, Streptomycin. Evaluation of antibacterial activity of methanolic, ethanolic, aqueous extract prepared from powdered seed of Trachyspermum ammi and Piper nigrum determined by disc diffusion method in Muller Hinton
agar against bacteria. 25µg/ml seed extract was tested against the bacteria and compared with antibiotics 10µg/disc. Zone of inhibition was measured and calculated. The result showed that Trachyspermum ammi and Piper nigrum extract had good antibacterial activity.
